5M9G
Human angiogenin PD variant K54R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.904, 118.755, 37.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.500 - 2.280 |
| R-factor | 0.22506 |
| Rwork | 0.220 |
| R-free | 0.27057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.500 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 8730 | |
| <I/σ(I)> | 6.7 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 6.1 |
| CC(1/2) | 0.994 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2 M Na/K tartrate 0.1 M HEPES 3% propanol 20% PEG 8000 |
