5M9F
Structure of the two C-terminal domains of bacteriophage K gp144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.690, 81.311, 154.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.800 |
| R-factor | 0.16352 |
| Rwork | 0.161 |
| R-free | 0.20620 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | 0.737 |
| Number of reflections | 59974 | |
| <I/σ(I)> | 8.6 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.8 | 6 |
| CC(1/2) | 0.995 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES pH 7.5, 12% w/v PEG 4000, 100 mM sodium acetate |
