5M97
Structure of the Mal3 EB1-like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-04-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.380, 37.910, 101.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.520 - 1.330 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.21180 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.519 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.520 | 1.378 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.092 | 1.963 |
| Number of reflections | 32150 | |
| <I/σ(I)> | 16.19 | 1.15 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 12.5 | 10.6 |
| CC(1/2) | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M NaCl, 30% MPD and 0.1 M sodium acetate at pH4.6 |
