5M95

STAPHYLOCOCCUS CAPITIS DIVALENT METAL ION TRANSPORTER (DMT) IN COMPLEX WITH MANGANESE

Replaces:  4WGW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.8940
Spacegroup name	P 31 2 1
Unit cell lengths	114.170, 114.170, 257.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 3.400
R-factor	0.253
Rwork	0.252
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4WGV
RMSD bond length	0.003
RMSD bond angle	0.694
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.600
High resolution limit [Å]	3.400	3.400
Rmerge	0.081	1.270
Number of reflections	27350
<I/σ(I)>	19	2.4
Completeness [%]	99.9	100
Redundancy	9.6	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	24 % PEG 400, 200 MM CACL2, 50 MM HEPES, PH 7.4