5M93
Crystal structure of SdeA-modified ubiquitin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.596, 81.073, 51.287 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 49.370 - 1.793 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.23104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.038 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 1.857 |
| High resolution limit [Å] | 1.793 | 1.793 |
| Rmerge | 0.122 | 0.796 |
| Number of reflections | 22956 | |
| <I/σ(I)> | 8.24 | 1.38 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.995 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M sodium acetate pH 4 - 5.5, 0.2M lithium sulfate and 30% PEG 8000 |
