5M8T

Crystal structure of human tyrosinase related protein 1 (T391V-R374S-Y362F) in complex with tropolone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	89.732, 140.605, 191.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.747 - 2.350
R-factor	0.185
Rwork	0.183
R-free	0.22540
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	48.750
High resolution limit [Å]	2.350
Number of reflections	100975
<I/σ(I)>	11.9
Completeness [%]	99.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000