5M8P
Crystal structure of human tyrosinase related protein 1 in complex with tyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.516, 140.322, 191.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.919 - 2.800 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.26200 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.760 |
High resolution limit [Å] | 2.800 |
Number of reflections | 59734 |
<I/σ(I)> | 7.6 |
Completeness [%] | 99.4 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000 |