5M8O

Crystal structure of human tyrosinase related protein 1 in complex with tropolone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	89.688, 140.202, 191.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.694 - 2.500
R-factor	0.2067
Rwork	0.204
R-free	0.25120
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	48.694
High resolution limit [Å]	2.500
Number of reflections	83673
<I/σ(I)>	6
Completeness [%]	99.8
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000