5M88
Spliceosome component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.867, 103.013, 94.105 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 47.005 - 2.800 |
| R-factor | 0.2517 |
| Rwork | 0.250 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | In-house model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.000 | 47.000 | 2.660 |
| High resolution limit [Å] | 2.540 | 8.810 | 2.540 |
| Rmerge | 0.058 | 0.036 | 1.783 |
| Rmeas | 0.064 | 0.040 | 1.990 |
| Rpim | 0.028 | 0.018 | 0.868 |
| Total number of observations | 65996 | 1585 | 7718 |
| Number of reflections | 13027 | 334 | 1531 |
| <I/σ(I)> | 11.1 | 36.8 | 1 |
| Completeness [%] | 99.2 | 99.4 | 96 |
| Redundancy | 5.1 | 4.7 | 5 |
| CC(1/2) | 0.999 | 0.999 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5 0.4 M KCl 45% Pentaerythritol Proxalate |
