5M87
Crystal structure of Eremococcus coleocola manganese transporter
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-02-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0, 0.9794, 0.9796, 0.9173 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.200, 81.650, 96.230 |
Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.300 |
R-factor | 0.2344 |
Rwork | 0.232 |
R-free | 0.27710 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.529 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmeas | 0.051 | 1.646 |
Number of reflections | 16757 | |
<I/σ(I)> | 26.6 | 1.8 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 13.6 | |
CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 50 mM Tris-HCl pH 8.6 22,8% PEG 400 (v/v) |