5M86
Crystal Structure of the Thermoplasma acidophilum Protein Ta1207
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97901,0.97916,0.97670 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.639, 112.821, 221.328 |
| Unit cell angles | 90.00, 118.70, 90.00 |
Refinement procedure
| Resolution | 29.658 - 2.400 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.20260 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | RESOLVE (2.13) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 2.430 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.077 | 0.703 |
| Rmeas | 0.090 | |
| Rpim | 0.048 | |
| Total number of observations | 663454 | |
| Number of reflections | 191535 | |
| <I/σ(I)> | 13.6 | 2.1 |
| Completeness [%] | 98.7 | 88.4 |
| Redundancy | 3.5 | 3.2 |
| CC(1/2) | 0.997 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 50 mM MES pH 6.0, 200 mM Ca-diacetate and 20%(w/v) PEG3350 |
