5M85

Three-dimensional structure of the intermediate state of GAF3 from Slr1393 of Synechocystis sp. PCC6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.954
Spacegroup name	P 65
Unit cell lengths	75.101, 75.101, 71.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.040 - 2.100
R-factor	0.17213
Rwork	0.170
R-free	0.22233
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dfx
RMSD bond length	0.019
RMSD bond angle	2.430
Data reduction software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.210	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	13390
<I/σ(I)>	33.34	5.96
Completeness [%]	99.6	98.2
Redundancy	20.07	19.2
CC(1/2)	1.000	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	0.1 M NaCl, 0.1 M LiSO4, 0.1 M CAPSO pH 9.5, 12% PEG 4000