5M82
Three-dimensional structure of the photoproduct state of GAF3 from Slr1393 of Synechocystis sp. PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976252 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.286, 75.286, 71.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.200 - 1.860 |
| R-factor | 0.19596 |
| Rwork | 0.194 |
| R-free | 0.22872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m85 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.376 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.200 | 1.980 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 18191 | |
| <I/σ(I)> | 25.79 | 3.37 |
| Completeness [%] | 94.4 | 78.9 |
| Redundancy | 3.89 | 3.07 |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 0.1 M NaCl, 0.1 M CAPSO pH 9.5, 0.175 M LiSO4, 14% PEG 4000 |
