5M7W

Translation initiation factor 4E in complex with (SP)-m2(7,2'O)GppSpG mRNA 5' cap analog (beta-S-ARCA D2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	38.048, 38.032, 147.340
Unit cell angles	95.98, 88.31, 103.47

Refinement procedure

Resolution	48.845 - 1.970
R-factor	0.215
Rwork	0.213
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	1.970	1.970
Rmerge	0.064	0.355
Number of reflections	54201
<I/σ(I)>	8.15	1.98
Completeness [%]	95.7	94.1
Redundancy	1.782	1.726
CC(1/2)	0.996	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	5% PEG 8000, 9% ethylene glycol, 0.03M ethylene glycols, 0.1M Trisma/bicine pH 8.5