5M7Q
Engineering the Thermostability of Nanobodies - NbD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 49.058, 49.058, 225.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.690 - 1.800 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.24856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w6x |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.047 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.690 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.479 | |
| Number of reflections | 30508 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.9 |
| CC(1/2) | 0.999 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290.15 | 100 mM MES, pH 6.5, 1.6 M magnesium sulfate heptahydrate |
