5M7L

Blastochloris viridis photosynthetic reaction center synchrotron structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	57.600, 82.900, 382.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.502 - 3.600
R-factor	0.2442
Rwork	0.242
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cas
RMSD bond length	0.006
RMSD bond angle	1.184
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.502
High resolution limit [Å]	3.580
Number of reflections	22398
<I/σ(I)>	4.1
Completeness [%]	98.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Melted monoolein was thoroughly mixed in a ratio of 3:2 (v/v) with 0.1M HEPES, pH 7.5, 0.1 % LDAO until a viscous, transparent LCP was obtained. The formed phase was then transferred into a glass vial and sponge-phase-inducing solution (1:4 ratio) was added containing 16% Jeffamine M-600, 1M HEPES pH 7.9, 0.7 M Ammonium sulphate, 2.5% 1,2,3-Heptanetriol, which swell the cubic phase to sponge phase. After phase separation overnight, the upper phase (sponge phase) was harvested. Crystals were grown using batch crystallization in the lipidic-sponge phase. Equal amount of sponge phase and protein were mixed with 2/5 (v/v) of 1.2 M Tris-sodium citrate and allowed to incubate for several weeks.