5M7K
Blastochloris viridis photosynthetic reaction center - RC_vir_xfel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2011-02-19 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.32 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.900, 84.800, 384.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.438 - 3.500 |
R-factor | 0.254 |
Rwork | 0.253 |
R-free | 0.27380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cas |
RMSD bond length | 0.003 |
RMSD bond angle | 0.712 |
Data scaling software | CrystFEL (0.6.2) |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.000 |
High resolution limit [Å] | 3.500 |
Number of reflections | 25004 |
<I/σ(I)> | 3.5 |
Completeness [%] | 99.4 |
Redundancy | 59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | Melted monoolein was thoroughly mixed in a ratio of 3:2 (v/v) with 0.1M HEPES, pH 7.5, 0.1 % LDAO until a viscous, transparent LCP was obtained. The formed phase was then transferred into a glass vial and sponge-phase-inducing solution (1:4 ratio) was added containing 16% Jeffamine M-600, 1M HEPES pH 7.9, 0.7 M Ammonium sulphate, 2.5% 1,2,3-Heptanetriol, which swell the cubic phase to sponge phase. After phase separation overnight, the upper phase (sponge phase) was harvested. Crystals were grown using batch crystallization in the lipidic-sponge phase. Equal amount of sponge phase and protein were mixed with 2/5 (v/v) of 1.2 M Tris-sodium citrate and allowed to incubate for several weeks. |