5M7I
Crystal structure of GH125 1,6-alpha-mannosidase mutant from Clostridium perfringens in complex with 1,6-alpha-mannobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.296, 43.734, 85.407 |
| Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
| Resolution | 60.860 - 2.100 |
| R-factor | 0.19452 |
| Rwork | 0.192 |
| R-free | 0.24698 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.860 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.160 | 0.840 |
| Number of reflections | 24135 | |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 91.6 | 57.2 |
| Redundancy | 2.9 | 2.6 |
| CC(1/2) | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 200 mM MgCl2, 100 mM HEPES pH7.0, 27% polyethylene glycol 3350 |
