5M72
Structure of the human SRP68-72 protein-binding domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972422 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.597, 52.269, 62.863 |
| Unit cell angles | 90.00, 118.22, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.600 |
| R-factor | 0.1446 |
| Rwork | 0.142 |
| R-free | 0.18410 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | |
| High resolution limit [Å] | 1.600 | |
| Rmerge | 0.057 | 0.550 |
| Number of reflections | 25085 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M K2SO4 20% (w/v) PEG 3350 |
