5M6K
Crystal structure of nitrophorin 7 E27V mutant from Rhodnius prolixus with imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.260, 66.645, 38.494 |
| Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
| Resolution | 23.858 - 1.600 |
| R-factor | 0.1615 |
| Rwork | 0.159 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xmc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (2011_08_24_1020) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.560 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.058 | 0.581 |
| Number of reflections | 83100 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | NaOAc/HOAc |
