5M68
AT-rich DNA dodecamer with extra helical guanine-nickel coordination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 32 |
| Unit cell lengths | 44.780, 44.780, 99.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.780 - 2.640 |
| R-factor | 0.22824 |
| Rwork | 0.227 |
| R-free | 0.24690 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.847 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 | 0.637 |
| Number of reflections | 6470 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 97.0 | 69.5 |
| Redundancy | 5.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | Sodium Cacodylate Buffer pH 6.5, MPD 50%, Nickel II, Magnesium II |
