5M61
Clathrin heavy chain N-terminal domain bound to an extended amphiphysin clathrin-box motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.120, 131.150, 77.830 |
| Unit cell angles | 90.00, 115.35, 90.00 |
Refinement procedure
| Resolution | 39.660 - 1.840 |
| R-factor | 0.16247 |
| Rwork | 0.161 |
| R-free | 0.18153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.084 | 1.232 |
| Number of reflections | 102809 | |
| <I/σ(I)> | 12.8 | 1.5 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.8 | 6.2 |
| CC(1/2) | 0.999 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 1 uL protein:peptide mix (14 mg/mL protein, 3.4 mM peptide) plus 2 uL of 1.04 M sodium malonate pH 7.1, 0.2 M sodium perchlorate equilibrated against a 200 uL reservoir of 1.15 M sodium malonate pH 7.1 |
