5M5U
Clathrin heavy chain N-terminal domain bound to a clathrin-box motif from hepatitis D virus large antigen (clade 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.230, 131.190, 77.870 |
| Unit cell angles | 90.00, 115.61, 90.00 |
Refinement procedure
| Resolution | 48.370 - 2.150 |
| R-factor | 0.17696 |
| Rwork | 0.175 |
| R-free | 0.20704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.101 | 0.930 |
| Number of reflections | 63339 | |
| <I/σ(I)> | 9.6 | 1.5 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.996 | 0.501 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 400 nL protein:peptide mix (14 mg/mL NTD and 3.4 mM) plus 200 nL reservoir equilibrated against a 80 uL reservoir of 1.21 M sodium malonate pH 7.0 |
