5M58
Crystal structure of CouO, a C-methyltransferase from Streptomyces rishiriensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0507 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.070, 82.950, 76.880 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.050 |
| R-factor | 0.20662 |
| Rwork | 0.203 |
| R-free | 0.26448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.442 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.980 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.130 | 0.250 |
| Number of reflections | 25206 | |
| <I/σ(I)> | 6 | 2.5 |
| Completeness [%] | 97.9 | 94.2 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2.3 mg/ml CouO 12.5%(w/v) PEG 1000, 12.5%(w/v) PEG 3350, 12.5%(v/v) MPD, 0.03 M of each halide (sodium fluoride, sodium bromide and sodium iodide), 0.1 M MES/imidazole pH 6.5 |
