5M4Y

Crystal structure of the Sec3/Sso2 complex at 2.20 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	101.145, 135.803, 185.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.978 - 2.200
R-factor	0.2295
Rwork	0.229
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a58 1IFO
RMSD bond length	0.002
RMSD bond angle	0.422
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	62754
<I/σ(I)>	13.2
Completeness [%]	99.4
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M HEPES (pH 7.0), 0.2 M NaCl, and 25% (w/v) PEG 3350