5M4S
Transcription factor TFIIA as a single chain protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2010-10-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.312, 123.312, 34.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.360 - 2.380 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.088 |
| Data reduction software | XSCALE |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 2.520 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 12483 | |
| <I/σ(I)> | 11.5 | 2.72 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mM Tris (8.0), 1mM DTT, 0.5mM EDTA and 25mM NaCl |
