5M4R
Structural tuning of CD81LEL (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.120, 104.970, 65.010 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 53.093 - 3.100 |
| R-factor | 0.2267 |
| Rwork | 0.226 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.958 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.100 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.074 | 0.525 |
| Number of reflections | 15510 | |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 98.7 | 99.4 |
| Redundancy | 3.3 | 3.5 |
| CC(1/2) | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 294.15 | Protein: ~10mg/ml Buffer: 0.1 M NaCitrate pH 4.0, 0.8 M (NH4)2 SO4 |
