Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99983 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.221, 104.075, 114.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.850 - 2.380 |
| R-factor | 0.2271 |
| Rwork | 0.224 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nkx |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.850 | 2.500 | |
| High resolution limit [Å] | 2.380 | 40.000 | 2.380 |
| Rmerge | 0.066 | 0.037 | 0.598 |
| Number of reflections | 35499 | ||
| <I/σ(I)> | 16.76 | 35.98 | 3.11 |
| Completeness [%] | 99.7 | 27.3 | 99.9 |
| Redundancy | 6.4 | ||
| CC(1/2) | 0.999 | 1.000 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277.15 | 0.17 M Ammonium acetate; 0.085 M Sodium citrate pH 5.6; 25.5%(w/v) PEG 4000; 15%(v/v) Glycerol |
