5M2W
Structure of nanobody nb18 raised against TssK from E. coli T6SS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-31 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.390, 53.390, 88.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.100 - 1.500 |
| R-factor | 0.2032 |
| Rwork | 0.203 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.088 | 0.785 |
| Number of reflections | 38858 | |
| <I/σ(I)> | 7.8 | 1.6 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.994 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.75 | 293 | 100 nL of 13 mg per ml nb18 protein solution with 100 nL of 0.2 M Li2SO4, 0.1 M NaAc pH 4.75, 30% PEG8000 |
