5M2R
The Structure of the Ycf54 A9G mutant protein from Synechocystis sp. PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.200, 46.130, 119.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.540 - 1.500 |
| R-factor | 0.18503 |
| Rwork | 0.182 |
| R-free | 0.24331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m2p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.540 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.770 |
| Rpim | 0.040 | 0.350 |
| Number of reflections | 18771 | |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 6.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1M trisodium citrate, 2.4M ammonium sulphate |
