5M2P
The Structure of the Ycf54 protein from Synechocystis sp. PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.730, 45.930, 119.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.650 - 1.330 |
| R-factor | 0.15034 |
| Rwork | 0.149 |
| R-free | 0.17147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hze |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.402 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.650 | |
| High resolution limit [Å] | 1.330 | |
| Rmerge | 0.520 | |
| Number of reflections | 27322 | |
| <I/σ(I)> | 16.5 | 2.2 |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2M ammonium fluoride, 2.4M ammonium sulphate |
