5M28

Maltodextrin binding protein MalE1 from L. casei BL23 bound to maltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	54.273, 82.645, 84.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.443 - 1.079
R-factor	0.1292
Rwork	0.129
R-free	0.14080
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.924
Data reduction software	XDS (November 11, 2013)
Data scaling software	XDS (November 11, 2013)
Phasing software	SHELXDE (2013/2)
Refinement software	PHENIX ((1.10.1-2155_1168: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.730	1.120
High resolution limit [Å]	1.079	1.079
Rmerge	0.073	1.102
Number of reflections	155473
<I/σ(I)>	25.3	2.5
Completeness [%]	94.8	74.32
Redundancy	16.13	13.36
CC(1/2)	1.000	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 mM Na-Hepes, pH 7.0, 20-25% Jeffamine ED2003, pH 7.0, 10 mM SrCl2