5M1X
Crystal structure of S. cerevisiae Rfa1 N-OB domain mutant (K45E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.620, 115.350, 69.030 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 44.260 - 1.800 |
| R-factor | 0.1669 |
| Rwork | 0.164 |
| R-free | 0.21360 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.260 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.530 | |
| Number of reflections | 80762 | |
| <I/σ(I)> | 8.02 | 1.74 |
| Completeness [%] | 95.4 | 86.2 |
| Redundancy | 2.4 | 2.3 |
| CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM ammonium fluoride 20 % (w/v) PEG 3350 |
