5M1M

Crystal structure of matrix protein 1 from Influenza C virus (strain C/Ann Arbor/1/1950)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.93928
Spacegroup name	C 1 2 1
Unit cell lengths	66.398, 26.841, 77.224
Unit cell angles	90.00, 108.40, 90.00

Refinement procedure

Resolution	31.502 - 1.500
R-factor	0.1894
Rwork	0.188
R-free	0.21950
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	20888
<I/σ(I)>	12.1	2.4
Completeness [%]	99.2	99.3
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100mM Tris pH8.0, 200mM MgCl2 and25% PEG3350