5M1C
Crystal structure of N-terminally tagged apo-UbiD from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92818 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 196.435, 196.435, 108.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 98.410 - 2.750 |
R-factor | 0.21499 |
Rwork | 0.213 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2idb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.410 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.118 | 0.936 |
Number of reflections | 52741 | |
<I/σ(I)> | 12.9 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.1 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 mM SPG pH 8, 25% w/v PEG 1500 |