5M11

Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	115.520, 115.520, 139.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.460 - 2.900
R-factor	0.185
Rwork	0.183
R-free	0.23810
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.500	3.180	3.050
High resolution limit [Å]	2.900	3.100	2.900
Rmerge	0.108	0.976	0.913
Number of reflections	21600
<I/σ(I)>	20	1.8	3.4
Completeness [%]	99.9	98.6	99.7
Redundancy	12.1	2.8	12.1
CC(1/2)	0.999	0.584	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	8 % polyethylene glycol 10000, 0.2 M zinc acetate, 0.008 M calcium chloride 0.1 M sodium cacodylate, pH 6.5
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	8 % polyethylene glycol 10000, 0.2 M zinc acetate, 0.008 M calcium chloride 0.1 M sodium cacodylate, pH 6.5