5M0X
Structure of apo structure of GH36 alpha-galactosidase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.960, 95.780, 97.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.090 - 1.800 |
| R-factor | 0.18845 |
| Rwork | 0.186 |
| R-free | 0.23128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zy9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.795 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.090 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.706 |
| Number of reflections | 59066 | |
| <I/σ(I)> | 14.9 | 2.6 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M magnesium sulfate, 20% (w/v) PEG 3350 |
