5M0P
Crystal structure of cytochrome P450 OleT F79A in complex with arachidonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.880, 110.120, 164.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.120 - 1.950 |
| R-factor | 0.15937 |
| Rwork | 0.157 |
| R-free | 0.20120 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.270 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.120 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.057 | 0.842 |
| Number of reflections | 66360 | |
| <I/σ(I)> | 8.6 | 1.8 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M MgCl2, 0.1 M HEPES, 20% PEG 6000, pH 7.5 |
