5M0K
CRYSTAL STRUCTURE of endo-1,4-beta-xylanase from Cellulomonas flavigena
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.151, 122.879, 40.904 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 46.321 - 2.590 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 21576 |
| <I/σ(I)> | 5.21 |
| Completeness [%] | 99.4 |
| Redundancy | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 15% PEG 4K, 50 mM Sodium Citrate, 20 mM Sodium chloride, 20 mM Ammonium Sulfate |
