5M0H
Crystal structure of the central flexible region of ASH1 mRNA E3-localization element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.320, 64.320, 75.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.481 - 2.650 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.073 | 0.800 |
| Number of reflections | 4920 | |
| <I/σ(I)> | 19.93 | 2.27 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.24 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 50 mM MES, pH 6.0, 5 % (w/v) PEG 4000, 5 mM MgSO4 |
