5M04
Structure of ObgE from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07812 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.590, 83.000, 177.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.730 - 1.850 |
| R-factor | 0.20007 |
| Rwork | 0.198 |
| R-free | 0.23881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lnz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.763 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.730 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 2.326 |
| Rmeas | 0.098 | |
| Number of reflections | 41090 | |
| <I/σ(I)> | 9.67 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.81 | 6.97 |
| CC(1/2) | 0.998 | 0.489 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 16% (w/v) polyethylene glycol (PEG) 6000, 15% (v/v) 2-propanol and 100 mM Sodium Citrate/HCl pH 5.6. |
