5M02
Crystal structure of murine P14 TCR / H-2Db with PF, modified gp33 peptide from LCMV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 255.110, 46.500, 89.020 |
Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
Resolution | 127.140 - 1.750 |
R-factor | 0.18926 |
Rwork | 0.188 |
R-free | 0.21386 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7u |
RMSD bond length | 0.011 |
RMSD bond angle | 1.522 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 128.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.043 | |
Number of reflections | 103816 | |
<I/σ(I)> | 13.5 | 1.7 |
Completeness [%] | 98.5 | 97.3 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% PEG 6000, 0.1M Tris HCl pH 8.0 |