5LZK
Structure of the domain of unknown function DUF1669 from human FAM83B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.498, 73.959, 63.081 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 47.832 - 1.575 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.18890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4urj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1682) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.832 | 1.581 |
| High resolution limit [Å] | 1.575 | 1.575 |
| Rmerge | 0.064 | 0.665 |
| Number of reflections | 46738 | |
| <I/σ(I)> | 11.2 | 2 |
| Completeness [%] | 99.8 | 96 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.998 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 29% PEG3350 0.1M bis-tris-propane pH 8.0 0.2M sodium iodide 10% ethylene glycol |
