5LZ1

Crystal structure of human ACBD3 GOLD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.282724
Spacegroup name	P 43 21 2
Unit cell lengths	56.210, 56.210, 163.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.746 - 2.000
R-factor	0.2195
Rwork	0.218
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1olm
RMSD bond length	0.003
RMSD bond angle	0.896
Data reduction software	XDS (version Oct 15, 2015)
Data scaling software	XDS (version Oct 15, 2015)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.750	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.059	1.349
Number of reflections	18582
<I/σ(I)>	18.66	1.43
Completeness [%]	99.8	99.45
Redundancy	7.7	7.9
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM Tris pH 8.8, 25% w/v PEG 3.350, 20% glycerol, 20mM 2-propanol/1,2 -propanediol/1,3-propanediol/1-butanol/1,4-butanediol/1,6-hexanediol