5LYS
The crystal structure of 7SK 5'-hairpin - Gold derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.04014 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.320, 47.830, 69.230 |
| Unit cell angles | 90.00, 105.54, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.320 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Theoretical model of RNA generated with RNAcomposer |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.098 | 1.327 |
| Number of reflections | 23716 | |
| <I/σ(I)> | 9.4 | 1.12 |
| Completeness [%] | 93.7 | 61.9 |
| Redundancy | 3.9 | 3.56 |
| CC(1/2) | 0.998 | 0.469 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 1000, 50 mM Tris pH 7.5, 50 to 75 mM NaCl, 50 mM MgCl2, 3% DMSO |
