5LYN
Structure of the Tpr Domain of Sgt2 in complex with yeast Ssa1 peptide fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 298.15 |
| Detector technology | PIXEL |
| Collection date | 2016-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.490, 61.090, 55.250 |
| Unit cell angles | 90.00, 108.81, 90.00 |
Refinement procedure
| Resolution | 33.586 - 2.000 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vyi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.680 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.590 | 33.590 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.084 | 0.056 | 0.392 |
| Rmeas | 0.091 | ||
| Rpim | 0.036 | ||
| Total number of observations | 126863 | ||
| Number of reflections | 19295 | ||
| <I/σ(I)> | 16.4 | ||
| Completeness [%] | 99.1 | 96.7 | 98 |
| Redundancy | 6.6 | 5.8 | 6.4 |
| CC(1/2) | 0.997 | 0.995 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.2 | 293.15 | 0.2 M Zinc Acetate, 30% PEG 3350 |
