5LYN

Structure of the Tpr Domain of Sgt2 in complex with yeast Ssa1 peptide fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2016-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	45.490, 61.090, 55.250
Unit cell angles	90.00, 108.81, 90.00

Refinement procedure

Resolution	33.586 - 2.000
R-factor	0.1598
Rwork	0.158
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vyi
RMSD bond length	0.006
RMSD bond angle	0.680
Data reduction software	xia2
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.590	33.590	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.084	0.056	0.392
Rmeas	0.091
Rpim	0.036
Total number of observations	126863
Number of reflections	19295
<I/σ(I)>	16.4
Completeness [%]	99.1	96.7	98
Redundancy	6.6	5.8	6.4
CC(1/2)	0.997	0.995	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.2	293.15	0.2 M Zinc Acetate, 30% PEG 3350