5LY8
Structure of the CBM2 module of Lactobacillus casei BL23 phage J-1 evolved Dit.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.020, 63.670, 74.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.440 - 1.280 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CBM2 CsI phased |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.432 | |
| Number of reflections | 60896 | |
| <I/σ(I)> | 28.7 | 3.8 |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 7 | 5.9 |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | protein at 8 mg/mL in 0.8-1.2 M sodium citrate as precipitant with 10mM sodium borate at pH 8.9-9.7. |
