5LXZ

W288A mutant of GlxA from Streptomyces lividans: Cu-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	70.510, 80.870, 140.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.480 - 1.490
R-factor	0.164
Rwork	0.163
R-free	0.18120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4unm
RMSD bond length	0.013
RMSD bond angle	1.655
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.480	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.090	0.754
Rmeas	0.106
Rpim	0.053
Total number of observations	480382
Number of reflections	130534
<I/σ(I)>	7.6	2
Completeness [%]	99.5	99.5
Redundancy	3.7	3.8
CC(1/2)	0.991	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	20% PEG 20000, 0.1M sodium acetate pH 5 15 mg/ml protein