5LXY
Structure of the minimal RBM7 - ZCCHC8 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.771, 66.575, 111.912 |
| Unit cell angles | 90.00, 126.57, 90.00 |
Refinement procedure
| Resolution | 71.790 - 2.850 |
| Rwork | 0.262 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lxr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.147 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.790 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.061 | |
| Number of reflections | 24933 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.2 | |
| CC(1/2) | 0.995 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.1 M Bis-Tris-Propane 0.2 M NaBr 0.1 M Sodium malonate 20% (w/v) PEG3350 |
