5LXV
Crystal structure of Ruminococcus flavefaciens scaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.590, 66.730, 109.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.000 - 2.400 |
R-factor | 0.2188 |
Rwork | 0.217 |
R-free | 0.25980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ccl |
RMSD bond length | 0.005 |
RMSD bond angle | 0.949 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.590 | 59.590 | 2.500 |
High resolution limit [Å] | 2.400 | 8.650 | 2.400 |
Rmerge | 0.080 | 0.033 | 0.321 |
Number of reflections | 17195 | ||
<I/σ(I)> | 51.7 | ||
Completeness [%] | 97.6 | 98.1 | 99.3 |
Redundancy | 4 | 4.1 | 4 |
CC(1/2) | 0.985 | 0.964 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M potassium thiocyanate, 30% w/v PEG 2000 MME |